A new approach identifies reproducible volatile organic compounds for lung cancer diagnosis

The researchers have developed a multimedia approach (MMA) to identify reproducible volatile organic compounds (VOCs) in lung cancer cells. This study, published in Analytical Chemistry, provides new insights into non-invasive lung cancer diagnosis through VOCs.

VOCs of human odor have been an important focus of health research, particularly for screening for non-invasive lung cancer. Despite decades of research on VOCs in vomiting, there has been no consensus on reliable biomarkers, and in vitro cancer cell culture analysis has not achieved inconsistent results.

To address this, the team used three different medium combinations of RPMI 1640, DMEM, and HAM F12 in chromatography mass spectroscopy (GC-MS) for untargeted VOC analysis. I proposed.

“The newly proposed MMA allowed us to find some important VOCs that our team could reliably separate from normal lung cells (A549),” said team member Dr. Ge Dianlong.

Dozens of differential VOCs have been identified using traditional single medium approaches (SMA), but only two VOCs (methylbutanol isomers) are reproducible under MMA, and the cancerous A549 cells are It was shown to be low levels. Further validation by target detection of subcutaneous and primary tumor tissues in animal models confirmed these findings.

“This new approach is like creating a universal “fingerprint” of cancer cells, providing hope for more accurate and non-invasive detection of lung cancer in the future,” adds Dr. GE. Ta. “These findings also help advance tumor gas biopsies and also help improve the diagnosis of traditional Chinese herbal medicines (TCM).”

This research was led by Professor Chu Yang Nan of the Institute of Physical Sciences at the Chinese Academy of Sciences